Novel, high-quality surface plasmon resonance microscopy

Transmission surface plasmon resonance microscopy

Novel, Cost-effective, High-quality Surface Plasmon Resonance and Fluorescence Microscope

We demonstrate a novel, low-cost method of building a surface plasmon resonance and fluorescence (SPRF) microscope with high quality SPR and evanescent-field fluorescence imaging using a laser pointer and an oscillating scatterer. SPR Microscopy and SPR fluorescence provide two detection modalities in a highly compact inexpensive package. Applications include point-of-care diagnostics with sens...

Multiphoton plasmon-resonance microscopy.

A novel method for detection of noble-metal nanoparticles by their nonlinear optical properties is presented and applied for specific labeling of cellular organelles. When illuminated by laser light in resonance with their plasmon frequency these nanoparticles generate an enhanced multiphoton signal. This enhanced signal is measured to obtain a depth-resolved image in a laser scanning microscop...

Surface plasmon resonance biosensor

Imaging surface plasmon resonance

The central theme of this thesis is the use of imaging Surface Plasmon Resonance (iSPR) as a tool in the characterization of surfaces with laterally varying properties. Within the scope of this work, an instrument for iSPR analysis was designed and built. SPR is a very sensitive technique for monitoring changes in optical properties in the immediate vicinity of a sensor surface, which is very u...